Abstract:
48 cases of diffuse large cell lymphomas (DLCLs) from Zurich
were morphologically classified according to the Kiel
Classification of non-Hodgkins lymphomas (NHL) and
immunologically into large centrocytic 7 cases, centroblastic
23; B immunoblastic 10, peripheral T cell lymphomas (PTCLs)
6, and true histiocytic sarcoma 2 cases. 73% of the cases were
of B cell, 23% of T cell and 4% of true histiocytic origin.
Immunohistochemically Anti-leucocyte common antigen (LCA)
PD7/26, 2B11 showed concordant immunoreactivity with 66% of
DLCLs. LN2 and MB2 proved reliable B cell markers by reacting
with 70% and 74% of B cell DLCLs respectively. None of the T
cell lymphomas showed reactivity with these antibodies. UCHL1
proved a better T cell marker than MT1 by reacting with 72% of
PTCLs against 45% of the latter. In addition 2 true
histiocytic sarcomas were identified by immunophenotyping
using appropriate markers. Light chain polytypic antibodies
did not prove useful in detecting B cell DLCLs.
Comparatively, immunophenotyping of 32 cases of DLCLs from Dar
es Salaam showed 90% to be of B cell and 10% of T cell origin.
Antibody PD 7/26, 2B11 showed immunoreactivity with 50% of the
cases. LN1 and LN2 showed much lower percentage of
immunoreactivity with B cell DLCLs than Zurich series. 2 of
the 3 T cell DLCLs reacted with UCHL1.
