Use of the GenoType® MTBDRplus assay to assess drug resistance of Mycobacterium tuberculosis isolates from patients in rural Uganda

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dc.contributor.author Bazira, J.
dc.contributor.author Asiimwe, B.B.
dc.contributor.author Joloba, M.L.
dc.contributor.author Bwanga, F.
dc.contributor.author Matee, M.I.
dc.date.accessioned 2013-02-11T12:12:56Z
dc.date.available 2013-02-11T12:12:56Z
dc.date.issued 2010
dc.identifier.citation Bazira, J., Asiimwe, B. B., Joloba, M. L., Bwanga, F., & Matee, M. I. (2010). Use of the GenoType® MTBDRplus assay to assess drug resistance of Mycobacterium tuberculosis isolates from patients in rural Uganda. BMC clinical pathology, 10(1), 5.
dc.identifier.issn 1472-6890
dc.identifier.other doi:10.1186/1472-6890-10-5
dc.identifier.uri http://hdl.handle.net/123456789/275
dc.description.abstract Background: Drug resistance levels and patterns among Mycobacterium tuberculosis isolates from newly diagnosed and previously treated tuberculosis patients in Mbarara Uganda were investigated. Methods: We enrolled, consecutively, all newly diagnosed and previously treated smear-positive TB patients aged ≥ 18 years. Isolates were tested for drug resistance against rifampicin (RIF) and isoniazid (INH) using the Genotype® MDRTBplus assay and results were compared with those obtained by the indirect proportion method on Lowenstein-Jensen media. HIV testing was performed using two rapid HIV tests. Results: A total of 125 isolates from 167 TB suspects with a mean age 33.7 years and HIV prevalence of 67.9% (55/81) were analysed. A majority (92.8%) of the participants were newly presenting while only 7.2% were retreatment cases. Resistance mutations to either RIF or INH were detected in 6.4% of the total isolates. Multidrug resistance, INH and RIF resistance was 1.6%, 3.2% and 4.8%, respectively. The rpob gene mutations seen in the sample were D516V, S531L, H526Y H526 D and D516V, while one strain had a Δ1 mutation in the wild type probes. There were three strains with katG (codon 315) gene mutations while only one strain showed the inhA promoter region gene mutation. Conclusion: The TB resistance rate in Mbarara is relatively low. The GenoType® MTBDRplus assay can be used for rapid screening of MDR-TB in this setting. en_GB
dc.language.iso en en_GB
dc.publisher BioMed Central en_GB
dc.relation.ispartofseries BMC Clinical Pathology;10:5
dc.subject GenoType en_GB
dc.subject MTBDRplus en_GB
dc.subject Mycobacterium tuberculosis en_GB
dc.subject Uganda en_GB
dc.title Use of the GenoType® MTBDRplus assay to assess drug resistance of Mycobacterium tuberculosis isolates from patients in rural Uganda en_GB
dc.type Article en_GB


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