Kaposi’s sarcoma herpesvirus load in biopsies of cutaneous and oral Kaposi’s sarcoma lesions

Show simple item record

dc.contributor.author Pak, F.
dc.contributor.author Mwakigonja, A.R.
dc.contributor.author Kokhaei, P.
dc.contributor.author Hosseinzadeh, N.
dc.contributor.author Pyakurel, P.
dc.contributor.author Kaaya, E.
dc.contributor.author Bogdanovic, G.
dc.contributor.author Selivanova, G.
dc.contributor.author Biberfeld, P.
dc.date.accessioned 2013-02-15T13:24:14Z
dc.date.available 2013-02-15T13:24:14Z
dc.date.issued 2007
dc.identifier.issn 2007.05.023
dc.identifier.uri http://hdl.handle.net/123456789/497
dc.description.abstract Objectives: To evaluate human herpesvirus 8/Kaposi’s sarcoma associated herpesvirus (HHV-8/KSHV) viral load in diagnostic, (formalin fixed, paraffinised) biopsies and patient serum during tumour progression of oral and cutaneous AIDS-related Kaposi’s sarcoma (AKS), and endemic Kaposi’s sarcoma (EKS) by a sensitive and specific quantitative real time polymerase chain reaction (qRT-PCR) assay. Study design: Eighty six biopsies of both AKS (oral and cutaneous AKS, 68) and EKS (cutaneous EKS, 18) were evaluated by qRT-PCR and immunohistochemistry (IHC). The viral load in human tumour tissue and serum of some individual patients were compared. Results: Higher viral load as well as frequency of latency-associated nuclear antigen (LANA)+ tumour spindle cells (SC) and number of LANA granules per SC was found in oral AKS compared to cutaneous AKS. Although few cases were available, serum viral load appeared to decrease compared to tumour tissue during KS progression. Conclusions: The higher viral load in oral rather than cutaneous AKS is consistent with the well recognised reservoir function of the oral mucosa. Decrease of serum HHV-8 load during KS progression may indicate decreased virus release and/or increased virus clearance. en_GB
dc.language.iso en en_GB
dc.publisher ELSEVIER en_GB
dc.relation.ispartofseries European Journal of Cancer 43(2007 )1877 –1882
dc.subject Kaposi’s sarcoma en_GB
dc.subject KSHV/HHV-8 en_GB
dc.subject Real time PCR en_GB
dc.subject Immunohistochemistry en_GB
dc.title Kaposi’s sarcoma herpesvirus load in biopsies of cutaneous and oral Kaposi’s sarcoma lesions en_GB
dc.type Article en_GB


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search MUHAS IR


Advanced Search

Browse

My Account